RNA
Part:BBa_K2611000:Design
Designed by: Yuanhan Yang Group: iGEM18_SCU-China (2018-10-17)
sgRNA (spacer J23101-GFP)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Should carefully notice the complementary of the sgRNA and its target sequence (part "spacer J23101-GFP"BBa_K2611001).
Source
This sgRNA is produced by part "sgRNA generator"(BBa_K1689000). The spacer sequence is selected from a reported sequence in which the function of this spacer has been proved. And the backbone is BBa_K1689000. Reference: Bikard, D., Jiang, W., Samai, P., Hochschild, A., Zhang, F., & Marraffini, L. A. (2013). Programmable repression and activation of bacterial gene expression using an engineered crispr-cas system. Nucleic Acids Research, 41(15), 7429-7437.