RNA

Part:BBa_K2611000:Design

Designed by: Yuanhan Yang   Group: iGEM18_SCU-China   (2018-10-17)


sgRNA (spacer J23101-GFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Should carefully notice the complementary of the sgRNA and its target sequence (part "spacer J23101-GFP"BBa_K2611001).


Source

This sgRNA is produced by part "sgRNA generator"(BBa_K1689000). The spacer sequence is selected from a reported sequence in which the function of this spacer has been proved. And the backbone is BBa_K1689000. Reference: Bikard, D., Jiang, W., Samai, P., Hochschild, A., Zhang, F., & Marraffini, L. A. (2013). Programmable repression and activation of bacterial gene expression using an engineered crispr-cas system. Nucleic Acids Research, 41(15), 7429-7437.


References